[HTML][HTML] C4d fixing, luminex binding antibodies—a new tool for prediction of graft failure after heart transplantation
JD Smith, IM Hamour, NR Banner, ML Rose - American Journal of …, 2007 - Elsevier
The standard method to detect pretransplant antibodies has been the complement
dependent cytotoxicity (CDC) test of donor leukocytes. Solid phase assays to detect HLA
antibodies in pretransplant serum reveal a greater number of sensitized patients, but their
clinical impact is less certain. Here we have developed a method of detecting C4d fixing
HLA antibodies on Luminex beads. Pretransplant serum from 565 cardiac transplant
patients was retrospectively tested for the presence of HLA antibodies using CDC, HLA …
dependent cytotoxicity (CDC) test of donor leukocytes. Solid phase assays to detect HLA
antibodies in pretransplant serum reveal a greater number of sensitized patients, but their
clinical impact is less certain. Here we have developed a method of detecting C4d fixing
HLA antibodies on Luminex beads. Pretransplant serum from 565 cardiac transplant
patients was retrospectively tested for the presence of HLA antibodies using CDC, HLA …