Publisher Summary This chapter discusses the specific synthesis of deoxyribonucleic acid
(DNA) in vitro through the medium of a polymerase-catalyzed chain reaction. A method
whereby a nucleic acid sequence can be exponentially amplified in vitro is described in the
chapter. The same method can be used to alter the amplified sequence or to append new
sequence information to it. It is necessary that the ends of the sequence be known in
sufficient detail that oligonucleotides can be synthesized, which will hybridize to them and …