Thioredoxin increases exocytosis by denitrosylating N-ethylmaleimide-sensitive factor
T Ito, M Yamakuchi, CJ Lowenstein - Journal of biological chemistry, 2011 - ASBMB
Exocytosis involves membrane fusion between granules and the plasma membrane. Nitric
oxide (NO) inhibits exocytosis by chemically modifying N-ethylmaleimide-sensitive factor
(NSF), a key component of the exocytic machinery. However, cells recover the ability to
release messenger molecules within hours of exposure to NO through unknown
mechanisms. We now identify thioredoxin (TRX1) as a denitrosylase that reverses NO
inhibition of exocytosis. Endogenously synthesized NO increases S-nitrosylated NSF levels …
oxide (NO) inhibits exocytosis by chemically modifying N-ethylmaleimide-sensitive factor
(NSF), a key component of the exocytic machinery. However, cells recover the ability to
release messenger molecules within hours of exposure to NO through unknown
mechanisms. We now identify thioredoxin (TRX1) as a denitrosylase that reverses NO
inhibition of exocytosis. Endogenously synthesized NO increases S-nitrosylated NSF levels …
