Identification of a Third Metalloprotease Toxin Gene in Extraintestinal Isolates of Bacteroides fragilis
GT Chung, AA Franco, S Wu, GE Rhie… - Infection and …, 1999 - Am Soc Microbiol
GT Chung, AA Franco, S Wu, GE Rhie, R Cheng, HB Oh, CL Sears
Infection and immunity, 1999•Am Soc MicrobiolTo further understand the epidemiology of enterotoxigenic Bacteroides fragilis (ETBF), 89
extraintestinal B. fragilis strains from Seoul, Korea, were examined for secretion of B. fragilis
toxin (BFT) by the HT29/C1 biologic assay and for the B. fragilis toxin gene (bft) by colony
blot hybridization and PCR. Complete agreement between the three techniques was found.
Overall, 34 B. fragilis strains (38%) were identified as ETBF. Eleven of the 34 ETBF strains
(32%) expressed a new isoform of BFT (Korea-BFT). This new isoform is more related to BFT …
extraintestinal B. fragilis strains from Seoul, Korea, were examined for secretion of B. fragilis
toxin (BFT) by the HT29/C1 biologic assay and for the B. fragilis toxin gene (bft) by colony
blot hybridization and PCR. Complete agreement between the three techniques was found.
Overall, 34 B. fragilis strains (38%) were identified as ETBF. Eleven of the 34 ETBF strains
(32%) expressed a new isoform of BFT (Korea-BFT). This new isoform is more related to BFT …
Abstract
To further understand the epidemiology of enterotoxigenicBacteroides fragilis (ETBF), 89 extraintestinal B. fragilis strains from Seoul, Korea, were examined for secretion of B. fragilis toxin (BFT) by the HT29/C1 biologic assay and for the B. fragilis toxin gene (bft) by colony blot hybridization and PCR. Complete agreement between the three techniques was found. Overall, 34 B. fragilis strains (38%) were identified as ETBF. Eleven of the 34 ETBF strains (32%) expressed a new isoform of BFT (Korea-BFT). This new isoform is more related to BFT-2 than to BFT-1. Like BFT-1 and BFT-2, Korea-BFT cleaves E-cadherin, the zonula adherens protein.
American Society for Microbiology