The human tumor necrosis factor (TNF) receptor-associated factor 1 gene (TRAF1) is up-regulated by cytokines of the TNF ligand family and modulates TNF-induced …
R Schwenzer, K Siemienski, S Liptay… - Journal of Biological …, 1999 - ASBMB
To understand how the TNF receptor-associated factor 1 (TRAF1) is transcriptionally
regulated, in vitroDNA binding assays, promoter-reporter gene assays, and RNase
protection assays were performed with the human TRAF1 gene. Binding of NF-κB to three of
five putative binding sites within the human TRAF1 promoter was found in electrophoretic
mobility shift assay studies, and analysis of TRAF1 gene promoter luciferase constructs
confirmed the functional importance of these elements. Moreover, triggering of TNF-R1 …
regulated, in vitroDNA binding assays, promoter-reporter gene assays, and RNase
protection assays were performed with the human TRAF1 gene. Binding of NF-κB to three of
five putative binding sites within the human TRAF1 promoter was found in electrophoretic
mobility shift assay studies, and analysis of TRAF1 gene promoter luciferase constructs
confirmed the functional importance of these elements. Moreover, triggering of TNF-R1 …
