The calcium-dependent association and functional coupling of calmodulin with myocardial phospholipase A2: implications for cardiac cycle-dependent alterations in …
MJ Wolf, RW Gross - Journal of Biological Chemistry, 1996 - ASBMB
Herein we demonstrate the calcium-dependent regulation of myocardial phospholipase A 2
activity, which is mediated by a cytosolic protein constituent that can be chromatographically
resolved from, and subsequently reconstituted with, purified myocardial phospholipase A 2.
Purification of this protein by sequential column chromatographies revealed an 18-kDa
doublet, which was identified as calmodulin by Western blotting, calcium-dependent
precipitation with W-7 agarose beads, and reconstitution of calcium-mediated …
activity, which is mediated by a cytosolic protein constituent that can be chromatographically
resolved from, and subsequently reconstituted with, purified myocardial phospholipase A 2.
Purification of this protein by sequential column chromatographies revealed an 18-kDa
doublet, which was identified as calmodulin by Western blotting, calcium-dependent
precipitation with W-7 agarose beads, and reconstitution of calcium-mediated …
