[HTML][HTML] Role of neurotrophin-4/5 in neural cell death during retinal development and ischemic retinal injury in vivo

C Harada, T Harada, HMA Quah… - … & visual science, 2005 - tvst.arvojournals.org
C Harada, T Harada, HMA Quah, K Namekata, K Yoshida, S Ohno, K Tanaka, LF Parada
Investigative ophthalmology & visual science, 2005tvst.arvojournals.org
purpose. Neurotrophin (NT)-4/5 and brain-derived neurotrophic factor (BDNF) mediate cell
survival through TrkB, a high-affinity tyrosine kinase receptor, and may prevent neural cell
death in various pathologic conditions. This study was conducted to investigate the function
of NT-4/5 in neural cell death during retinal development and ischemic retinal injury.
methods. Retinal development in wild-type, NT-4/5 knockout (KO), and NT-4/5: BDNF
double-KO mice was histologically examined from postnatal day 0 (P0) to P90. Ischemic …
Abstract
purpose. Neurotrophin (NT)-4/5 and brain-derived neurotrophic factor (BDNF) mediate cell survival through TrkB, a high-affinity tyrosine kinase receptor, and may prevent neural cell death in various pathologic conditions. This study was conducted to investigate the function of NT-4/5 in neural cell death during retinal development and ischemic retinal injury.
methods. Retinal development in wild-type, NT-4/5 knockout (KO), and NT-4/5: BDNF double-KO mice was histologically examined from postnatal day 0 (P0) to P90. Ischemic retinal injury was performed at P42, and NT-4/5 mRNA expression level and the extent of retinal cell death was quantitatively examined.
results. Real-time PCR analysis revealed increased NT-4/5 mRNA expression in the ischemic retina. In the NT-4/5 KO mouse, retinal development and structure were normal, but the strain was susceptible to ischemic injury on P42. In contrast, NT-4/5: BDNF double-KO mice showed delayed retinal development and died before P42.
conclusions. These results suggest that NT-4/5, in combination with other trophic factors, is involved in the postnatal survival of retinal neurons during both development and degeneration.
ARVO Journals