Use of an 18O2 Inhalation Technique and Mass Isotopomer Distribution Analysis to Study Oxygenation of Cholesterol in Rat: EVIDENCE FOR IN VIVO FORMATION …
O Breuer, I Björkhem - Journal of Biological Chemistry, 1995 - ASBMB
Cholesterol oxidation products (oxysterols) have been detected in many different tissues,
often at concentrations 10 3 to 10 4 times lower than cholesterol. This constitutes a
considerable risk of quantitation errors, since even a minor oxidation of cholesterol during
sample processing would yield a substantial increase of oxysterol levels. It has therefore
been suggested that some of the oxysterols do not occur in vivo and their detection in
tissues merely are artifacts produced in vitro. In the present work, an 18 O 2 inhalation …
often at concentrations 10 3 to 10 4 times lower than cholesterol. This constitutes a
considerable risk of quantitation errors, since even a minor oxidation of cholesterol during
sample processing would yield a substantial increase of oxysterol levels. It has therefore
been suggested that some of the oxysterols do not occur in vivo and their detection in
tissues merely are artifacts produced in vitro. In the present work, an 18 O 2 inhalation …
